Douglas Fraser

Children's Health & Therapeutics Dr. Douglas Fraser
Scientist
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Affiliations

Scientist, Division of Children's Health and Therapeutics, Children’s Health Research Institute
Consultant, Paediatrics Critical Care Medicine, Children’s Hospital, London Health Sciences Centre
Associate Professor, Departments of Paediatrics, Physiology & Pharmacology, and Clinical Neurological Sciences, Schulich School of Medicine & Dentistry, Western University
Clinician Scientist, Centre for Critical Illness Research, Lawson Health Research Institute
Director, Translational Research Centre

How my research helps children

Brain injury is a common reason for children to be admitted to the Critical Care Unit and is a major cause of death and long-term disability. The causes of brain injury in children are diverse and include trauma, stroke, metabolic disease, severe sepsis, and diabetic ketoacidosis. Despite the different origins of brain injury, there are commonalities in pathophysiological mechanisms. We are determining these key mechanisms of injury to the developing brain for development of neuroprotective therapeutics and we are determining prognostic indicators of brain injury severity to help stratify therapeutic interventions.

Research

Current Research Activities

Our research includes several mouse models of brain injury and collection of biological samples from children with acquired brain injury for laboratory analyses (blood and cerebrospinal fluid).

  1. Determining the mechanisms underlying acquired brain injury in trauma, diabetic ketoacidosis and severe sepsis/inflammation. This work includes mouse disease models complemented with use of human tissues for well-controlled in vitro laboratory studies.
  2. Determining the sensitivity and specificity for prognosticating severity of brain injury following trauma, diabetic ketoacidosis and severe sepsis/inflammation.

Paediatric Critical Illness/Trauma pathophysiology and epidemiology.

 

Current Research Team

Graduate Students:

  1. Ms. Taylor Close, MSc (CIHR Doctoral Scholarship)
  2. Mr. Chris Blom, BSc (Department of Paediatrics Graduate Student Scholarship)
  3. Ms. Claudia Augustine, BSc (Children's Health Foundation, Graduate Student Support)

Post-doctoral Fellows:

  1. Dr. Fukashi Serizawa, MD, PhD (Sendai, Japan)
  2. Dr. Tatsushi Omatsu, MD, PhD (Kyoto, Japan)

Research Assistant:

  1. Ms. Carolina Gillio-Meina, PhD (TRC)

Collaboration:

  1. Dr. Gedas Cepinskas, PhD (Scientist, Lawson Health Research Institute)
  2. Dr. Jason Gilliland, PhD (Associate Professor, UWO Geography)
  3. Ms. Tanya Charyk Stewart, MSc (Epidemiologist, LHSC Trauma Program)
  4. Canadian Critical Care Translational Biology Group (www.ccctbg.ca)

 

Future Research Plans

My research is strongly translational, with future plans of strengthening relationships between laboratory and clinical studies.  In addition, I am currently collaborating with several industry partners to develop bedside prognostic kits.

Awards & Grants

Awards & Grants

AMOSO Innovation Fund

SSMD Dean's Research Initiative Grant

Children's Health Foundation, Quality of Life

Lawson Health Research Institute (LHRI)

Collaborative Research Seed Grant

Nicolas and Regina Veenboer Foundation for medical research

Heart and Stroke Foundation

Ontario Brain Institute, Pilot Study

Canadian Institute of Health Research (CIHR)

Heart and Stroke Foundation

Children's Health Foundation (CHF)

Recent Publications

Publications

Translational Research: State-of-the-Art Reviews

Translational research in pediatrics III: Brochoalveolar lavage fluid
Rhadhakrishnan D, Yamashita C, Gillio-Meina C, Fraser DD
Pediatrics. 2014; 134(1):135-154

Translational research in pediatrics II: blood collection, processing, shipping and storage
Gillio-Meina C, Cepinskas G, Cecchini EL, Fraser DD
Pediatrics. 2013; 131(4): 754-766

Translational research in pediatrics: tissue sampling and bio-banking
Brisson A, Matsui D, Rieder MJ, Fraser DD
Pediatrics. 2012; 129(1):153-62

Traumatic Brain Injury

Traumatic injury elicits JNK-mediated human astrocyte retraction
Augustine C, Cepinskas G, Fraser DD
Neuroscience. 2014; 274C:1-10

Subarachnoid Hemorrhage Prevalence and Its Association with Short-Term Outcome in Pediatric Severe Traumatic Brain Injury
Hochstadter E, Charyk Stewart T, Alharfi I, Ranger A, Fraser DD
Neurocrit Care. 2014 Dec; 21(3):505-13

Brain injury biomarkers as outcome predictors after pediatric severe traumatic brain injury
Daoud H, Alharfi I, Qasem H, Charyk Stewart T, Fraser DD
Neurocritical Care. 2014; 20(3):427-35

An epidemiological profile of pediatric concussions: comparison of urban versus rural cohorts
Charyk Stewart T, Gilliland J, Fraser DD
Journal of Trauma and Acute Care Surgery. 2014; 76(3):736-42

Infection rates, fevers and associated factors in pediatric severe traumatic brain injury
Alharfi I, Charyk Stewart T, Alhelali I, Daoud H, Fraser DD
Journal of Neurotrauma. 2014; 31(5):452-8

Diabetic Ketoacidosis

CXCL1/CXCL8 chemokines (GROalpha/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro
Omatsu T, Cepinskas G, Patterson EK, Clarson C, Alharfi IM, Summers K, Couraud PO, Romero IA, Weksler B, Fraser DD
American Journal of Physiology: Endocrinology and Metabolism. 2014; 306(9):E1077-84

Diabetic ketoacidosis elicits systemic inflammation and cerebrovascular endothelial cell dysfunction in juvenile mice
Close TE, Cepinskas G, Omatsu T, Rose KE, Summers K, Fraser DD
Microcirculation. 2013; 20:534-543

Sepsis

Pre-treatment of human cerebrovascular endothelial cells with CO-releasing molecule-3 (CORM-3) interferes with JNK/AP-1 signaling and suppresses LPS-induced pro-adhesive phenotype
Serizawa F, Patterson E, Potter RF, Fraser DD, Cepinskas G
Microcirculation. 2015 Jan; 22(1):28-36

Th2-polarized invariant natural killer T cells reduce disease severity in acute intra-abdominal sepsis
Anantha RV, Mazzuca DM, Xu SX, Porcelli S, Welch I, Fraser DD, Martin CM, Mele T, Haeryfar SMM, McCormick JK
Clin Exp Immunol. 2014 Nov; 178(2):292-309

Carbon Monoxide Releasing Molecule-3 (CORM-3) Interferes with Myeloperoxidase (MPO) Catalytic Activity and Offers Protection Against MPO-Induced Vascular Endothelial Cell Activation/Dysfunction
Patterson E, Fraser DD, Capretta A, Potter RF, Cepinskas G
Free Radicals in Biology and Medicine. 2014; 70:167-73

Book Chapters

Trauma
F Serizawa, E Patterson, G Cepinskas, DD Fraser
In: Oxidative Stress in Applied Basic Research and Clinical Practice - Pediatric Disorders (eds. H Tsukahara, K Kaneko, RH Steinhorn)
Springer Science and Business Media. New York, NY. 2014

Troponin as a marker of clinically significant myocardial injury in Trauma
M Gharib, G Sangha, DD Fraser
In: Troponin: Informative Diagnostic Marker (ed. J-P Jin)
Nova Publishers Inc. Hauppauge, NY. 2014

Additional publications

Contact

Contact

Phone: (519) 685-8500, x58386 or 58052
Fax: (519) 685-8766
Email: douglas [dot] fraser [at] lhsc [dot] on [dot] ca
Website: https://www.schulich.uwo.ca/paediatrics/faculty/doug-fraser

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